Clonal analysis by ribotyping of Fusobacterium nucleatum isolates obtained from healthy young adults with optimal plaque control
Identifieur interne : 009984 ( Main/Exploration ); précédent : 009983; suivant : 009985Clonal analysis by ribotyping of Fusobacterium nucleatum isolates obtained from healthy young adults with optimal plaque control
Auteurs : G. Suchett-Kaye [France] ; D. Décoret [France] ; O. Barsotti [France]Source :
- Journal of Periodontal Research [ 0022-3484 ] ; 1998-05.
English descriptors
- KwdEn :
- Bacterial species, Bacterial strains, Baseline, Clin, Clin periodontol, Clonal, Clonal types, Dental students, Different clonal types, Diseased sites, Dzink, Endonuclease, Fnla, Fnla fnla, Fnlb, Fusobacterium, Fusobacterium nucleatum, Fusobacterium nucleaturn, Genetic heterogeneity, Gingival, Gingival inflammation, Gingival sulcus, Harboured, Healthy adults, Healthy individuals, Heterogeneity, High prevalence, Identical ribotype patterns, Identical ribotypes, Immunol, Implant, Microbiol, Nucleatum, Nucleatum clonal types, Nucleaturn, Nucleaturn clonal types, Nucleaturn ribotypes, Nucleaturn strains, Optimal plaque control, Oral cavity, Oral microbiol immunol, Outpatient, Periodontal, Periodontal disease, Periodontitis, Periodontol, Periodontopathic microorganisms, Plaque, Porphyromonas gingivalis, Positive subjects, Present study, Restriction endonuclease analysis, Restriction endonucleases, Results show, Ribotype, Ribotype patterns, Ribotypes, Ribotyping, Socransky, Students harboured, Subsp.
- Teeft :
- Bacterial species, Bacterial strains, Baseline, Clin, Clin periodontol, Clonal, Clonal types, Dental students, Different clonal types, Diseased sites, Dzink, Endonuclease, Fnla, Fnla fnla, Fnlb, Fusobacterium, Fusobacterium nucleatum, Fusobacterium nucleaturn, Genetic heterogeneity, Gingival, Gingival inflammation, Gingival sulcus, Harboured, Healthy adults, Healthy individuals, Heterogeneity, High prevalence, Identical ribotype patterns, Identical ribotypes, Immunol, Implant, Microbiol, Nucleatum, Nucleatum clonal types, Nucleaturn, Nucleaturn clonal types, Nucleaturn ribotypes, Nucleaturn strains, Optimal plaque control, Oral cavity, Oral microbiol immunol, Outpatient, Periodontal, Periodontal disease, Periodontitis, Periodontol, Periodontopathic microorganisms, Plaque, Porphyromonas gingivalis, Positive subjects, Present study, Restriction endonuclease analysis, Restriction endonucleases, Results show, Ribotype, Ribotype patterns, Ribotypes, Ribotyping, Socransky, Students harboured, Subsp.
Abstract
Fusobacterium nucleatum is a Gram‐negative anaerobic rod implicated in the pathogenesis of periodontal disease. However, this organism has also been frequently identified in high numbers in healthy adults. These observations suggest that the species may comprise different clonal types, some of which may participate in disease. The purpose of the present investigation was to use restriction endonuclease analysis (REA) and ribotyping to characterize F. nucleatum clonal types isolated from healthy young adults with optimal plaque control and investigate the stability of some of these clonal types. A group comprising 11 dental students and 11 dental outpatients with optimal plaque control was sampled. Clonal stability was investigated by sampling the dental student group at baseline and at 16 months. One hundred and thirty‐two clinical isolates of F. nucleatum were successfully recovered from 15/22 individuals. For the positive subjects, 29 different clonal types were identified by REA and ribotyping, each subject and site being colonized by 1–4 clonal types. For the dental students, 9 and 15 different clonal types were identified at baseline and 16 months, respectively. None of the students harboured identical clonal types at both sampling times. Our results show that ribotyping is a useful technique for monitoring the distributions of F. nucleatum clonal types and indicate that healthy individuals with optimal plaque control can be colonized by more than one F. nucleatum clonal type and that these clonal types appear to be unstable.
Url:
- https://api.istex.fr/document/0D85284D7D5EA241A1CB9C9480ACB56996127845/fulltext/pdf
- https://api.istex.fr/document/7EDD5A938D4B14C657B06112ADFF508C299B3ACC/fulltext/pdf
DOI: 10.1111/j.1600-0765.1998.tb02189.x
Affiliations:
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Le document en format XML
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Suchett-Kaye</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Etude des Interfaces en Odontologie, UFR d'Odontologie, Rue Guillaume Paradin, 69372 Lyon Cedex 08</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Correspondence address: G. Suchett‐Kaye, Laboratoire d'Etude des Interfaces en Odontologie, UFR d'Odontologie, Rue Guillaume Paradin 69372, Lyon Cedex 08</wicri:regionArea><wicri:noRegion>69372, Lyon Cedex 08</wicri:noRegion><wicri:noRegion>Lyon Cedex 08</wicri:noRegion></affiliation></author><author><name sortKey="Decoret, D" sort="Decoret, D" uniqKey="Decoret D" first="D." last="Décoret">D. Décoret</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Etude des Interfaces en Odontologie, UFR d'Odontologie, Rue Guillaume Paradin, 69372 Lyon Cedex 08</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Barsotti, O" sort="Barsotti, O" uniqKey="Barsotti O" first="O." last="Barsotti">O. Barsotti</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Etude des Interfaces en Odontologie, UFR d'Odontologie, Rue Guillaume Paradin, 69372 Lyon Cedex 08</wicri:regionArea><wicri:noRegion>69372 Lyon Cedex 08</wicri:noRegion><wicri:noRegion>69372 Lyon Cedex 08</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><biblScope unit="vol">33</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="179">179</biblScope><biblScope unit="page" to="186">186</biblScope><biblScope unit="page-count">8</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1998-05">1998-05</date></imprint><idno type="ISSN">0022-3484</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial species</term><term>Bacterial strains</term><term>Baseline</term><term>Clin</term><term>Clin periodontol</term><term>Clonal</term><term>Clonal types</term><term>Dental students</term><term>Different clonal types</term><term>Diseased sites</term><term>Dzink</term><term>Endonuclease</term><term>Fnla</term><term>Fnla fnla</term><term>Fnlb</term><term>Fusobacterium</term><term>Fusobacterium nucleatum</term><term>Fusobacterium nucleaturn</term><term>Genetic heterogeneity</term><term>Gingival</term><term>Gingival inflammation</term><term>Gingival sulcus</term><term>Harboured</term><term>Healthy adults</term><term>Healthy individuals</term><term>Heterogeneity</term><term>High prevalence</term><term>Identical ribotype patterns</term><term>Identical ribotypes</term><term>Immunol</term><term>Implant</term><term>Microbiol</term><term>Nucleatum</term><term>Nucleatum clonal types</term><term>Nucleaturn</term><term>Nucleaturn clonal types</term><term>Nucleaturn ribotypes</term><term>Nucleaturn strains</term><term>Optimal plaque control</term><term>Oral cavity</term><term>Oral microbiol immunol</term><term>Outpatient</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontitis</term><term>Periodontol</term><term>Periodontopathic microorganisms</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Positive subjects</term><term>Present study</term><term>Restriction endonuclease analysis</term><term>Restriction endonucleases</term><term>Results show</term><term>Ribotype</term><term>Ribotype patterns</term><term>Ribotypes</term><term>Ribotyping</term><term>Socransky</term><term>Students harboured</term><term>Subsp</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Bacterial species</term><term>Bacterial strains</term><term>Baseline</term><term>Clin</term><term>Clin periodontol</term><term>Clonal</term><term>Clonal types</term><term>Dental students</term><term>Different clonal types</term><term>Diseased sites</term><term>Dzink</term><term>Endonuclease</term><term>Fnla</term><term>Fnla fnla</term><term>Fnlb</term><term>Fusobacterium</term><term>Fusobacterium nucleatum</term><term>Fusobacterium nucleaturn</term><term>Genetic heterogeneity</term><term>Gingival</term><term>Gingival inflammation</term><term>Gingival sulcus</term><term>Harboured</term><term>Healthy adults</term><term>Healthy individuals</term><term>Heterogeneity</term><term>High prevalence</term><term>Identical ribotype patterns</term><term>Identical ribotypes</term><term>Immunol</term><term>Implant</term><term>Microbiol</term><term>Nucleatum</term><term>Nucleatum clonal types</term><term>Nucleaturn</term><term>Nucleaturn clonal types</term><term>Nucleaturn ribotypes</term><term>Nucleaturn strains</term><term>Optimal plaque control</term><term>Oral cavity</term><term>Oral microbiol immunol</term><term>Outpatient</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontitis</term><term>Periodontol</term><term>Periodontopathic microorganisms</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Positive subjects</term><term>Present study</term><term>Restriction endonuclease analysis</term><term>Restriction endonucleases</term><term>Results show</term><term>Ribotype</term><term>Ribotype patterns</term><term>Ribotypes</term><term>Ribotyping</term><term>Socransky</term><term>Students harboured</term><term>Subsp</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fusobacterium nucleatum is a Gram‐negative anaerobic rod implicated in the pathogenesis of periodontal disease. However, this organism has also been frequently identified in high numbers in healthy adults. These observations suggest that the species may comprise different clonal types, some of which may participate in disease. The purpose of the present investigation was to use restriction endonuclease analysis (REA) and ribotyping to characterize F. nucleatum clonal types isolated from healthy young adults with optimal plaque control and investigate the stability of some of these clonal types. A group comprising 11 dental students and 11 dental outpatients with optimal plaque control was sampled. Clonal stability was investigated by sampling the dental student group at baseline and at 16 months. One hundred and thirty‐two clinical isolates of F. nucleatum were successfully recovered from 15/22 individuals. For the positive subjects, 29 different clonal types were identified by REA and ribotyping, each subject and site being colonized by 1–4 clonal types. For the dental students, 9 and 15 different clonal types were identified at baseline and 16 months, respectively. None of the students harboured identical clonal types at both sampling times. Our results show that ribotyping is a useful technique for monitoring the distributions of F. nucleatum clonal types and indicate that healthy individuals with optimal plaque control can be colonized by more than one F. nucleatum clonal type and that these clonal types appear to be unstable.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Suchett Aye, G" sort="Suchett Aye, G" uniqKey="Suchett Aye G" first="G." last="Suchett-Kaye">G. Suchett-Kaye</name></region><name sortKey="Barsotti, O" sort="Barsotti, O" uniqKey="Barsotti O" first="O." last="Barsotti">O. Barsotti</name><name sortKey="Decoret, D" sort="Decoret, D" uniqKey="Decoret D" first="D." last="Décoret">D. Décoret</name><name sortKey="Suchett Aye, G" sort="Suchett Aye, G" uniqKey="Suchett Aye G" first="G." last="Suchett-Kaye">G. Suchett-Kaye</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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